Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes
In order to determine the genotype of the PXR A11156C polymorphism, we developed a real-time PCR fluorescence resonance energy transfer (FRET) assay. Although homozygotes could be discriminated unequivocally, separate melting peaks could not be obtained in putative heterozygote genotypes. To improve...
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| Veröffentlicht in: | The hematology journal : the official journal of the European Haematology Association 2005-04, Vol.5 (2), p.72-74 |
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| container_title | The hematology journal : the official journal of the European Haematology Association |
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| creator | Op den Buijsch, R A M de Vries, J E Loots, W J G Landt, O Wijnen, P A H M van Dieijen-Visser, M P Bekers, O |
| description | In order to determine the genotype of the PXR A11156C polymorphism, we developed a real-time PCR fluorescence resonance energy transfer (FRET) assay. Although homozygotes could be discriminated unequivocally, separate melting peaks could not be obtained in putative heterozygote genotypes. To improve the discriminative power of the sensor probe, two routes were followed. The first was to shorten the sensor probe and the second route was to modify the sensor probe with a locked nucleic acid (LNA) on the polymorphic position. |
| doi_str_mv | 10.1038/sj.tpj.6500299 |
| format | Article |
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| subjects | Alleles Biomedical and Life Sciences Biomedicine Fluorescence Resonance Energy Transfer Fluorescent Dyes Gene Expression Genotype Genotyping Human Genetics Humans Nucleic Acids - genetics Oncology perspective Pharmacotherapy Polymorphism, Genetic - genetics Psychopharmacology Receptors, Cytoplasmic and Nuclear - genetics Receptors, Steroid - genetics Reverse Transcriptase Polymerase Chain Reaction Temperature |
| title | Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes |
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