Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes

In order to determine the genotype of the PXR A11156C polymorphism, we developed a real-time PCR fluorescence resonance energy transfer (FRET) assay. Although homozygotes could be discriminated unequivocally, separate melting peaks could not be obtained in putative heterozygote genotypes. To improve the discriminative power of the sensor probe, two routes were followed. The first was to shorten the sensor probe and the second route was to modify the sensor probe with a locked nucleic acid (LNA) on the polymorphic position.