Light-Activated DNA Polymerase

Light-Activated DNA Polymerase

When the time is right: The widely applied Thermus aquaticus DNA polymerase was rendered light‐activatable by incorporation of the photocaged amino acid ortho‐nitrobenzyl tyrosine in place of a key tyrosine residue in the active site (see picture). As the modified enzyme was completely inactive unti...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2009-07, Vol.48 (32), p.5950-5953
Hauptverfasser: Chou, Chungjung, Young, Douglas D, Deiters, Alexander
Format: Artikel
Sprache:eng
Schlagworte:
Catalytic Domain
DNA polymerases
mutagenesis
Mutation
Nitrobenzenes - chemistry
non‐natural amino acids
photoactivation
Photolysis
Polymerase Chain Reaction
Taq Polymerase - chemistry
Taq Polymerase - metabolism
Thermus aquaticus
Tyrosine - chemistry
Ultraviolet Rays
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Beschreibung
Zusammenfassung:When the time is right: The widely applied Thermus aquaticus DNA polymerase was rendered light‐activatable by incorporation of the photocaged amino acid ortho‐nitrobenzyl tyrosine in place of a key tyrosine residue in the active site (see picture). As the modified enzyme was completely inactive until irradiated with UV light, temporal regulation of polymerase activity was possible.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200901115