Positively Charged Base Surrogate for Highly Stable “Base Pairing” through Electrostatic and Stacking Interactions

Positively Charged Base Surrogate for Highly Stable “Base Pairing” through Electrostatic and Stacking Interactions

“Base pairs” of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This “base pair” greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pa...

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Veröffentlicht in:Journal of the American Chemical Society 2009-07, Vol.131 (29), p.9928-9930
Hauptverfasser: Kashida, Hiromu, Ito, Hidehiro, Fujii, Taiga, Hayashi, Takamitsu, Asanuma, Hiroyuki
Format: Artikel
Sprache:eng
Schlagworte:
Amino Alcohols - chemistry
Base Pairing
Butylene Glycols - chemistry
Molecular Conformation
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Static Electricity
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container_end_page 9930
container_issue 29
container_start_page 9928
container_title Journal of the American Chemical Society
container_volume 131
creator Kashida, Hiromu
Ito, Hidehiro
Fujii, Taiga
Hayashi, Takamitsu
Asanuma, Hiroyuki
description “Base pairs” of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This “base pair” greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pairs. Further stabilization of the duplex was observed when the number of cationic dyes increased.
doi_str_mv 10.1021/ja9013002
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subjects Amino Alcohols - chemistry
Base Pairing
Butylene Glycols - chemistry
Molecular Conformation
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Static Electricity
title Positively Charged Base Surrogate for Highly Stable “Base Pairing” through Electrostatic and Stacking Interactions
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