Positively Charged Base Surrogate for Highly Stable “Base Pairing” through Electrostatic and Stacking Interactions

Positively Charged Base Surrogate for Highly Stable “Base Pairing” through Electrostatic and Stacking Interactions

“Base pairs” of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This “base pair” greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pa...

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Veröffentlicht in:Journal of the American Chemical Society 2009-07, Vol.131 (29), p.9928-9930
Hauptverfasser: Kashida, Hiromu, Ito, Hidehiro, Fujii, Taiga, Hayashi, Takamitsu, Asanuma, Hiroyuki
Format: Artikel
Sprache:eng
Schlagworte:
Amino Alcohols - chemistry
Base Pairing
Butylene Glycols - chemistry
Molecular Conformation
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Static Electricity
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Zusammenfassung:“Base pairs” of cationic dyes (p-methylstilbazole) were incorporated into oligodeoxyribonucleotides (ODNs). This “base pair” greatly stabilized the duplex through electrostatic and stacking interactions. The melting temperature of modified ODN was higher than those of neutral dyes and native base pairs. Further stabilization of the duplex was observed when the number of cationic dyes increased.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja9013002