Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival

The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS)...

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Veröffentlicht in:American journal of transplantation 2005-04, Vol.5 (4), p.746-756
Hauptverfasser: Alvarez, Cristiam M., Opelz, Gerhard, Giraldo, Mabel C., Pelzl, Steffen, Renner, Fabrice, Weimer, Rolf, Schmidt, Jan, Arbeláez, Mario, García, Luis F., Süsal, Caner
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container_end_page 756
container_issue 4
container_start_page 746
container_title American journal of transplantation
container_volume 5
creator Alvarez, Cristiam M.
Opelz, Gerhard
Giraldo, Mabel C.
Pelzl, Steffen
Renner, Fabrice
Weimer, Rolf
Schmidt, Jan
Arbeláez, Mario
García, Luis F.
Süsal, Caner
description The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS) and without immunosuppression (LTS‐NoIS). T cells of LTS patients exhibited strongly altered TCR Vß usage, including an increased frequency of oligoclonality and a decreased frequency of polyclonality. All 3 LTS‐NoIS and 12 of 16 LTS‐IS patients demonstrated oligoclonality in at least three or more TCR Vß families, and the frequency of oligoclonality in these patients was significantly higher as compared to patients with well‐functioning grafts at 3 years (p < 0.005 both), an uncomplicated course during the first year (p < 0.0001, both), acute rejection (p < 0.0001, both), chronic allograft nephropathy at 7 (p < 0.0001, both) or 13 years (p < 0.0001, both), dialysis patients (p < 0.0001, both) or healthy controls (p < 0.0001, both). In contrast to LTS patients, all other studied patient groups exhibited a polyclonal TCR repertoire. Our data indicate that TCR alteration is a common feature of long‐term allograft outcome, which might be explained by clonal deletion, exhaustion of alloreactive T cells or predominant expression of particular T‐cell subpopulations, such as regulatory T cells.
doi_str_mv 10.1111/j.1600-6143.2005.00756.x
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We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS) and without immunosuppression (LTS‐NoIS). T cells of LTS patients exhibited strongly altered TCR Vß usage, including an increased frequency of oligoclonality and a decreased frequency of polyclonality. All 3 LTS‐NoIS and 12 of 16 LTS‐IS patients demonstrated oligoclonality in at least three or more TCR Vß families, and the frequency of oligoclonality in these patients was significantly higher as compared to patients with well‐functioning grafts at 3 years (p < 0.005 both), an uncomplicated course during the first year (p < 0.0001, both), acute rejection (p < 0.0001, both), chronic allograft nephropathy at 7 (p < 0.0001, both) or 13 years (p < 0.0001, both), dialysis patients (p < 0.0001, both) or healthy controls (p < 0.0001, both). In contrast to LTS patients, all other studied patient groups exhibited a polyclonal TCR repertoire. Our data indicate that TCR alteration is a common feature of long‐term allograft outcome, which might be explained by clonal deletion, exhaustion of alloreactive T cells or predominant expression of particular T‐cell subpopulations, such as regulatory T cells.]]></description><identifier>ISSN: 1600-6135</identifier><identifier>EISSN: 1600-6143</identifier><identifier>DOI: 10.1111/j.1600-6143.2005.00756.x</identifier><identifier>PMID: 15760398</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; CDR3 spectrotyping ; Child ; Complementarity Determining Regions - chemistry ; Complementarity Determining Regions - genetics ; Complementarity Determining Regions - immunology ; Female ; GeneScan ; Graft Survival - immunology ; Humans ; Kidney Transplantation ; long‐term graft survival ; Male ; Middle Aged ; Receptors, Antigen, T-Cell - chemistry ; Receptors, Antigen, T-Cell - genetics ; Receptors, Antigen, T-Cell - immunology ; Renal Dialysis ; RT‐PCR ; TCR V‐beta ; Time Factors ; tolerance ; Transplantation, Homologous</subject><ispartof>American journal of transplantation, 2005-04, Vol.5 (4), p.746-756</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3666-da29c979d3e3431c7b1676483440e0402c033edc618f320dbb06a55ab7991dcf3</citedby><cites>FETCH-LOGICAL-c3666-da29c979d3e3431c7b1676483440e0402c033edc618f320dbb06a55ab7991dcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-6143.2005.00756.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-6143.2005.00756.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15760398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alvarez, Cristiam M.</creatorcontrib><creatorcontrib>Opelz, Gerhard</creatorcontrib><creatorcontrib>Giraldo, Mabel C.</creatorcontrib><creatorcontrib>Pelzl, Steffen</creatorcontrib><creatorcontrib>Renner, Fabrice</creatorcontrib><creatorcontrib>Weimer, Rolf</creatorcontrib><creatorcontrib>Schmidt, Jan</creatorcontrib><creatorcontrib>Arbeláez, Mario</creatorcontrib><creatorcontrib>García, Luis F.</creatorcontrib><creatorcontrib>Süsal, Caner</creatorcontrib><title>Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival</title><title>American journal of transplantation</title><addtitle>Am J Transplant</addtitle><description><![CDATA[The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS) and without immunosuppression (LTS‐NoIS). T cells of LTS patients exhibited strongly altered TCR Vß usage, including an increased frequency of oligoclonality and a decreased frequency of polyclonality. All 3 LTS‐NoIS and 12 of 16 LTS‐IS patients demonstrated oligoclonality in at least three or more TCR Vß families, and the frequency of oligoclonality in these patients was significantly higher as compared to patients with well‐functioning grafts at 3 years (p < 0.005 both), an uncomplicated course during the first year (p < 0.0001, both), acute rejection (p < 0.0001, both), chronic allograft nephropathy at 7 (p < 0.0001, both) or 13 years (p < 0.0001, both), dialysis patients (p < 0.0001, both) or healthy controls (p < 0.0001, both). In contrast to LTS patients, all other studied patient groups exhibited a polyclonal TCR repertoire. Our data indicate that TCR alteration is a common feature of long‐term allograft outcome, which might be explained by clonal deletion, exhaustion of alloreactive T cells or predominant expression of particular T‐cell subpopulations, such as regulatory T cells.]]></description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>CDR3 spectrotyping</subject><subject>Child</subject><subject>Complementarity Determining Regions - chemistry</subject><subject>Complementarity Determining Regions - genetics</subject><subject>Complementarity Determining Regions - immunology</subject><subject>Female</subject><subject>GeneScan</subject><subject>Graft Survival - immunology</subject><subject>Humans</subject><subject>Kidney Transplantation</subject><subject>long‐term graft survival</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Receptors, Antigen, T-Cell - chemistry</subject><subject>Receptors, Antigen, T-Cell - genetics</subject><subject>Receptors, Antigen, T-Cell - immunology</subject><subject>Renal Dialysis</subject><subject>RT‐PCR</subject><subject>TCR V‐beta</subject><subject>Time Factors</subject><subject>tolerance</subject><subject>Transplantation, Homologous</subject><issn>1600-6135</issn><issn>1600-6143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtOwzAURS0EoqWwBeQRs4bnOHESiUlVlZ8qgaCMLcdxSqokDnbSz4wlsEZWgkurMsUTX8nnvicfhDABj7hzvfAIAxgyElDPBwg9gChk3voI9Q8Px4dMwx46s3YBQCI_9k9Rj4QRA5rEfSQmS1F2oi10jXWOZ9-fX2NVlvhFSdW02rjQKNPqwiiLixo_O1TVrcWron3HU13PXWOmTOXAWpR4VJZ6bkTe4tfOLAs3_Byd5KK06mJ_D9Db7WQ2vh9On-4exqPpUFLG2DATfiKTKMmoogElMkoJi1gQ0yAABQH4EihVmWQkzqkPWZoCE2Eo0ihJSCZzOkBXu7mN0R-dsi2vCivdX0StdGc5i0IIA993YLwDpdHWGpXzxhSVMBtOgG_18gXfmuNbi3yrl__q5WtXvdzv6NJKZX_FvU8H3OyAVVGqzb8H89HjzAX6AwyMilM</recordid><startdate>200504</startdate><enddate>200504</enddate><creator>Alvarez, Cristiam M.</creator><creator>Opelz, Gerhard</creator><creator>Giraldo, Mabel C.</creator><creator>Pelzl, Steffen</creator><creator>Renner, Fabrice</creator><creator>Weimer, Rolf</creator><creator>Schmidt, Jan</creator><creator>Arbeláez, Mario</creator><creator>García, Luis F.</creator><creator>Süsal, Caner</creator><general>Munksgaard International Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200504</creationdate><title>Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival</title><author>Alvarez, Cristiam M. ; Opelz, Gerhard ; Giraldo, Mabel C. ; Pelzl, Steffen ; Renner, Fabrice ; Weimer, Rolf ; Schmidt, Jan ; Arbeláez, Mario ; García, Luis F. ; Süsal, Caner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3666-da29c979d3e3431c7b1676483440e0402c033edc618f320dbb06a55ab7991dcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>CDR3 spectrotyping</topic><topic>Child</topic><topic>Complementarity Determining Regions - chemistry</topic><topic>Complementarity Determining Regions - genetics</topic><topic>Complementarity Determining Regions - immunology</topic><topic>Female</topic><topic>GeneScan</topic><topic>Graft Survival - immunology</topic><topic>Humans</topic><topic>Kidney Transplantation</topic><topic>long‐term graft survival</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Receptors, Antigen, T-Cell - chemistry</topic><topic>Receptors, Antigen, T-Cell - genetics</topic><topic>Receptors, Antigen, T-Cell - immunology</topic><topic>Renal Dialysis</topic><topic>RT‐PCR</topic><topic>TCR V‐beta</topic><topic>Time Factors</topic><topic>tolerance</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alvarez, Cristiam M.</creatorcontrib><creatorcontrib>Opelz, Gerhard</creatorcontrib><creatorcontrib>Giraldo, Mabel C.</creatorcontrib><creatorcontrib>Pelzl, Steffen</creatorcontrib><creatorcontrib>Renner, Fabrice</creatorcontrib><creatorcontrib>Weimer, Rolf</creatorcontrib><creatorcontrib>Schmidt, Jan</creatorcontrib><creatorcontrib>Arbeláez, Mario</creatorcontrib><creatorcontrib>García, Luis F.</creatorcontrib><creatorcontrib>Süsal, Caner</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alvarez, Cristiam M.</au><au>Opelz, Gerhard</au><au>Giraldo, Mabel C.</au><au>Pelzl, Steffen</au><au>Renner, Fabrice</au><au>Weimer, Rolf</au><au>Schmidt, Jan</au><au>Arbeláez, Mario</au><au>García, Luis F.</au><au>Süsal, Caner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival</atitle><jtitle>American journal of transplantation</jtitle><addtitle>Am J Transplant</addtitle><date>2005-04</date><risdate>2005</risdate><volume>5</volume><issue>4</issue><spage>746</spage><epage>756</epage><pages>746-756</pages><issn>1600-6135</issn><eissn>1600-6143</eissn><abstract><![CDATA[The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS) and without immunosuppression (LTS‐NoIS). T cells of LTS patients exhibited strongly altered TCR Vß usage, including an increased frequency of oligoclonality and a decreased frequency of polyclonality. All 3 LTS‐NoIS and 12 of 16 LTS‐IS patients demonstrated oligoclonality in at least three or more TCR Vß families, and the frequency of oligoclonality in these patients was significantly higher as compared to patients with well‐functioning grafts at 3 years (p < 0.005 both), an uncomplicated course during the first year (p < 0.0001, both), acute rejection (p < 0.0001, both), chronic allograft nephropathy at 7 (p < 0.0001, both) or 13 years (p < 0.0001, both), dialysis patients (p < 0.0001, both) or healthy controls (p < 0.0001, both). In contrast to LTS patients, all other studied patient groups exhibited a polyclonal TCR repertoire. Our data indicate that TCR alteration is a common feature of long‐term allograft outcome, which might be explained by clonal deletion, exhaustion of alloreactive T cells or predominant expression of particular T‐cell subpopulations, such as regulatory T cells.]]></abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>15760398</pmid><doi>10.1111/j.1600-6143.2005.00756.x</doi><tpages>11</tpages></addata></record>
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subjects Adolescent
Adult
Aged
Aged, 80 and over
CDR3 spectrotyping
Child
Complementarity Determining Regions - chemistry
Complementarity Determining Regions - genetics
Complementarity Determining Regions - immunology
Female
GeneScan
Graft Survival - immunology
Humans
Kidney Transplantation
long‐term graft survival
Male
Middle Aged
Receptors, Antigen, T-Cell - chemistry
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - immunology
Renal Dialysis
RT‐PCR
TCR V‐beta
Time Factors
tolerance
Transplantation, Homologous
title Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival
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