Evaluation of T‐Cell Receptor Repertoires in Patients with Long‐Term Renal Allograft Survival
The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS)...
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Veröffentlicht in: | American journal of transplantation 2005-04, Vol.5 (4), p.746-756 |
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Sprache: | eng |
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Zusammenfassung: | The mechanisms underlying long‐term acceptance of kidney allografts in humans under minimal or no maintenance immunosuppression are poorly understood. We analyzed the T‐cell receptor (TCR) repertoires in circulating T cells of patients with long‐term (≥9 years) renal allograft survival with (LTS‐IS) and without immunosuppression (LTS‐NoIS). T cells of LTS patients exhibited strongly altered TCR Vß usage, including an increased frequency of oligoclonality and a decreased frequency of polyclonality. All 3 LTS‐NoIS and 12 of 16 LTS‐IS patients demonstrated oligoclonality in at least three or more TCR Vß families, and the frequency of oligoclonality in these patients was significantly higher as compared to patients with well‐functioning grafts at 3 years (p < 0.005 both), an uncomplicated course during the first year (p < 0.0001, both), acute rejection (p < 0.0001, both), chronic allograft nephropathy at 7 (p < 0.0001, both) or 13 years (p < 0.0001, both), dialysis patients (p < 0.0001, both) or healthy controls (p < 0.0001, both). In contrast to LTS patients, all other studied patient groups exhibited a polyclonal TCR repertoire. Our data indicate that TCR alteration is a common feature of long‐term allograft outcome, which might be explained by clonal deletion, exhaustion of alloreactive T cells or predominant expression of particular T‐cell subpopulations, such as regulatory T cells. |
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ISSN: | 1600-6135 1600-6143 |
DOI: | 10.1111/j.1600-6143.2005.00756.x |