Genotyping of Strawberry (Fragaria x ananassa Duch.) Cultivars by DNA Markers: Interlaboratory Study

Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria x ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were...

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Veröffentlicht in:	Journal of AOAC International 2009-05, Vol.92 (3), p.896-906
Hauptverfasser:	Kunihisa, Miyuki, Ueda, Hiroshi, Fukino, Nobuko, Matsumoto, Satoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences codominance Crops, Agricultural - genetics cultivars DNA Food industries Fragaria - genetics Fragaria ananassa Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Genetic aspects Genetic Markers genetic polymorphism Genetic research Genotype Identification and classification laboratories laboratory techniques Methods molecular sequence data nucleotide sequences pulsed-field gel electrophoresis ribotypes Sensitivity and Specificity Strawberries
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Zusammenfassung:	Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria x ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of 1 were >90%. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/92.3.896