Effect of Ca2+ and Mg2+ concentration in culture medium on the activation of recombinant factor IX produced in Chinese hamster ovary cells

Factor IX (FIX) plays an important role in the blood coagulation cascade. When Chinese hamster ovary (CHO) cells producing recombinant human FIX were cultivated using a serum-free medium (SFM) containing 1.12 mM of Ca(2+) and 0.82 mM of Mg(2+), a significant amount of active FIX (aFIX) was converted into undesirable activated FIX (FIXa) in the later phase of batch culture. In an effort to improve aFIX production from CHO cells, the effect of Ca(2+) and Mg(2+) concentrations in the culture medium on the activation of aFIX to FIXa was investigated using SFM with various concentrations of Ca(2+) and Mg(2+) in the range of 0-1.0 mM. The highest aFIX concentration of 1.36 IU/mL was obtained at 1.0 mM Ca(2+) and 1.0 mM Mg(2+), but the activation of aFIX to FIXa in the later phase of culture was rapid and significant. In contrast, at 0.5 mM Ca(2+) and 1.0 mM Mg(2+), the aFIX concentration of 1.33 IU/mL was obtained and did not decrease significantly in the later phase of culture. Taken together, lowering Ca(2+) concentration from 1.0 to 0.5 mM inhibits the activation of aFIX to FIXa in the later phase of culture, fortifying the robustness of downstream bioprocessing.