Efficient DNA Fingerprinting of Clostridium botulinum Types A, B, E, and F by Amplified Fragment Length Polymorphism Analysis

Amplified fragment length polymorphism (AFLP) analysis was applied to characterize 33 group I and 37 group II Clostridium botulinum strains. Four restriction enzyme and 30 primer combinations were screened to tailor the AFLP technique for optimal characterization of C. botulinum. The enzyme combinat...

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Veröffentlicht in:	Applied and Environmental Microbiology 2005-03, Vol.71 (3), p.1148-1154
Hauptverfasser:	Keto-Timonen, Riikka, Nevas, Mari, Korkeala, Hannu
Format:	Artikel
Sprache:	eng
Schlagworte:	amplified fragment length polymorphism Analysis Animals Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Clostridium botulinum Clostridium botulinum - classification Clostridium botulinum - genetics Clostridium botulinum - isolation & purification Clostridium botulinum A Clostridium botulinum B Clostridium botulinum E Clostridium botulinum F Clostridium botulinum type A - genetics Clostridium botulinum type B - genetics Clostridium botulinum type E - genetics Clostridium botulinum type F - genetics DNA fingerprinting DNA Fingerprinting - methods DNA fingerprints DNA, Bacterial - genetics Food Microbiology food pathogens Fundamental and applied biological sciences. Psychology Humans Microbiology Nucleic Acid Amplification Techniques - methods Phylogeny Polymorphism Polymorphism, Genetic
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Zusammenfassung:	Amplified fragment length polymorphism (AFLP) analysis was applied to characterize 33 group I and 37 group II Clostridium botulinum strains. Four restriction enzyme and 30 primer combinations were screened to tailor the AFLP technique for optimal characterization of C. botulinum. The enzyme combination HindIII and HpyCH4IV, with primers having one selective nucleotide apiece (Hind-C and Hpy-A), was selected. AFLP clearly differentiated between C. botulinum groups I and II; group-specific clusters showed
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.71.3.1148-1154.2005