Analysis of Protein-Protein Interactions by Using Droplet-Based Microfluidics

Every little drop: The KD values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodi...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2009-07, Vol.10 (10), p.1605-1611
Hauptverfasser:	Srisa-Art, Monpichar, Kang, Dong-Ku, Hong, Jongin, Park, Hyun, Leatherbarrow, Robin J, Edel, Joshua B, Chang, Soo-Ik, deMello, Andrew J
Format:	Artikel
Sprache:	eng
Schlagworte:	angiogenin angiogenin antibody Antibodies - immunology Cells, Cultured Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - chemistry Humans Kinetics microdroplets microfluidics Microfluidics - instrumentation Microfluidics - methods Protein Interaction Mapping - methods protein-protein interactions Ribonuclease, Pancreatic - analysis Ribonuclease, Pancreatic - immunology Ribonuclease, Pancreatic - metabolism Spectrometry, Fluorescence Thermodynamics
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Beschreibung
Zusammenfassung:	Every little drop: The KD values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200800841