Scaling of nucleic acid assays on microelectrophoresis array devices: High-dynamic range multi-gene readout from less than ten transcripts

In this paper we describe progress in using the prodigious data‐collecting ability of multilane microelectrophoresis instruments to bear on problems in scaled nucleic acid assays. We emphasize compound stacking and solid‐support loading as means to concentrate

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Bibliographische Detailangaben
Veröffentlicht in:	Electrophoresis 2009-06, Vol.30 (12), p.2090-2099
Hauptverfasser:	Ueberfeld, Joern, Ehrlich, Daniel J.
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Single-Stranded - analysis Electrophoresis, Microchip - instrumentation Electrophoresis, Microchip - methods Equipment Design Gene Expression Profiling - methods Microtechnology Oligonucleotide Array Sequence Analysis - methods PCR Polymerase Chain Reaction - methods Reaction Mapping Reproducibility of Results Sensitivity and Specificity Sequencing Solid-support
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Beschreibung
Zusammenfassung:	In this paper we describe progress in using the prodigious data‐collecting ability of multilane microelectrophoresis instruments to bear on problems in scaled nucleic acid assays. We emphasize compound stacking and solid‐support loading as means to concentrate
ISSN:	0173-0835 1522-2683
DOI:	10.1002/elps.200800774