Mutations in Subunits of the Escherichia coli Twin-arginine Translocase Block Function via Differing Effects on Translocation Activity or Tat Complex Structure
We have used a combination of blue-native (BN) gel electrophoresis and protein purification to analyze the effects of TatA or TatC mutations on the structures of the primary TatABC and multimeric TatA complexes in Escherichia coli. Expression of wild-type TatABC leads to the production of a single m...
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Veröffentlicht in: | Journal of molecular biology 2005-03, Vol.347 (2), p.453-463 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We have used a combination of blue-native (BN) gel electrophoresis and protein purification to analyze the effects of TatA or TatC mutations on the structures of the primary TatABC and multimeric TatA complexes in
Escherichia coli. Expression of wild-type TatABC leads to the production of a single major TatABC complex of 370
kDa and a heterogeneous set of TatA complexes of |
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ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/j.jmb.2005.01.026 |