Characterization of Human Skeletal Muscle Biopsy Samples Using Shotgun Proteomics

We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 p...

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Veröffentlicht in:	Journal of proteome research 2009-07, Vol.8 (7), p.3265-3277
Hauptverfasser:	Parker, Kenneth C, Walsh, Ronan J, Salajegheh, Mohammad, Amato, Anthony A, Krastins, Bryan, Sarracino, David A, Greenberg, Steven A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biopsy Chromatography, Liquid - methods Cluster Analysis Databases, Protein Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Humans Mass Spectrometry Muscle, Skeletal - pathology Peptides - chemistry Protein Array Analysis Proteome - analysis Proteome - metabolism Proteomics - methods Sarcomeres - metabolism Solubility
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Zusammenfassung:	We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr800873q