Portable microfluidic system for determination of urinary creatinine

A simple, low cost and portable microfluidic system based on a two-point alkaline picrate kinetic reaction has been developed for the determination of urinary creatinine. The creatinine reacts with picric acid under alkaline conditions, forming an orange-red colour, which is monitored on PDMS microc...

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Veröffentlicht in:Analytica chimica acta 2009-08, Vol.647 (1), p.78-83
Hauptverfasser: Songjaroen, Temsiri, Maturos, Thitima, Sappat, Aussawapong, Tuantranont, Adisorn, Laiwattanapaisal, Wanida
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Sprache:eng
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Zusammenfassung:A simple, low cost and portable microfluidic system based on a two-point alkaline picrate kinetic reaction has been developed for the determination of urinary creatinine. The creatinine reacts with picric acid under alkaline conditions, forming an orange-red colour, which is monitored on PDMS microchip using a portable miniature fibre optic spectrometer at 510 nm. A linear range was displayed from 0 to 40 mg L −1 creatinine ( r 2 = 0.997) with a detection limit of 3.3 mg L −1 (S/N = 3). On-chip absorbance signals are reproducible, with relative standard deviations (RSDs) of 7.1%, when evaluated with 20 mg L −1 creatinine ( n = 10). The standard curves in which the intra-run CVs (4.7–6.8%) and inter-run CVs (7.9%) obtained were performed on three different days and exhibited good reproducibility. The method was highly correlated with the conventional spectrophotometric method when real urine samples were evaluated ( r 2 = 0.948 ; n = 15).
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2009.05.014