PI(3,4,5)P3 Interactome
Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This l...
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| Veröffentlicht in: | Journal of proteome research 2009-07, Vol.8 (7), p.3712-3726 |
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| container_end_page | 3726 |
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| container_issue | 7 |
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| container_title | Journal of proteome research |
| container_volume | 8 |
| creator | Catimel, Bruno Yin, Meng-Xin Schieber, Christine Condron, Melanie Patsiouras, Heather Catimel, Jenny Robinson, Diane E. J. E Wong, Leon S.-M Nice, Edouard C Holmes, Andrew B Burgess, Antony W |
| description | Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC−MS/MS experiments. |
| doi_str_mv | 10.1021/pr900320a |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1535-3893 |
| ispartof | Journal of proteome research, 2009-07, Vol.8 (7), p.3712-3726 |
| issn | 1535-3893 1535-3907 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Carcinoma - metabolism Cell Line, Tumor Colonic Neoplasms - metabolism Computational Biology - methods Cytosol - metabolism Glutathione Transferase - metabolism Humans Liposomes - chemistry Mass Spectrometry - methods Models, Chemical Phosphates - chemistry Phosphatidylinositol Phosphates - chemistry Phosphatidylinositol Phosphates - metabolism Protein Interaction Mapping Proteome Proteomics - methods |
| title | PI(3,4,5)P3 Interactome |
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