PI(3,4,5)P3 Interactome

PI(3,4,5)P3 Interactome

Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This l...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of proteome research 2009-07, Vol.8 (7), p.3712-3726
Hauptverfasser: Catimel, Bruno, Yin, Meng-Xin, Schieber, Christine, Condron, Melanie, Patsiouras, Heather, Catimel, Jenny, Robinson, Diane E. J. E, Wong, Leon S.-M, Nice, Edouard C, Holmes, Andrew B, Burgess, Antony W
Format: Artikel
Sprache:eng
Schlagworte:
Carcinoma - metabolism
Cell Line, Tumor
Colonic Neoplasms - metabolism
Computational Biology - methods
Cytosol - metabolism
Glutathione Transferase - metabolism
Humans
Liposomes - chemistry
Mass Spectrometry - methods
Models, Chemical
Phosphates - chemistry
Phosphatidylinositol Phosphates - chemistry
Phosphatidylinositol Phosphates - metabolism
Protein Interaction Mapping
Proteome
Proteomics - methods
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC−MS/MS experiments.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr900320a