Spheroid array of fetal mouse liver cells constructed on a PEG-gel micropatterned surface: upregulation of hepatic functions by co-culture with nonparenchymal liver cells

Spheroid array of fetal mouse liver cells constructed on a PEG-gel micropatterned surface: upregulation of hepatic functions by co-culture with nonparenchymal liver cells

A spheroid array of fetal mouse liver cells, which comprise various immature cells, was constructed on a PEG-gel micropatterned surface and its hepatic activity and degree of differentiation induction were significantly upregulated by co-culture with nonparenchymal liver cells as feeder-cells.

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Veröffentlicht in:Lab on a chip 2009-01, Vol.9 (14), p.1991-1993
Hauptverfasser: Kojima, Ryota, Yoshimoto, Keitaro, Takahashi, Emiko, Ichino, Masahiro, Miyoshi, Hirotoshi, Nagasaki, Yukio
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Cell Differentiation
Coculture Techniques
Cytochrome P-450 CYP1A2 - metabolism
Gels
Hepatocytes - cytology
Hepatocytes - metabolism
Liver - cytology
Mice
Microarray Analysis - methods
Polyethylene Glycols - chemistry
Spheroids, Cellular - cytology
Spheroids, Cellular - metabolism
Surface Properties
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Beschreibung
Zusammenfassung:A spheroid array of fetal mouse liver cells, which comprise various immature cells, was constructed on a PEG-gel micropatterned surface and its hepatic activity and degree of differentiation induction were significantly upregulated by co-culture with nonparenchymal liver cells as feeder-cells.
ISSN:1473-0197
1473-0189
DOI:10.1039/b903388b