Proteomic profiling of human stem cells derived from umbilical cord blood
CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or i...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-03, Vol.328 (4), p.968-972 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Zenzmaier, Christoph Gesslbauer, Bernd Grobuschek, Nina Jandrositz, Anita Preisegger, Karl-Heinz Kungl, Andreas J. |
| description | CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified. |
| doi_str_mv | 10.1016/j.bbrc.2005.01.044 |
| format | Article |
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Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.01.044</identifier><identifier>PMID: 15707972</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>2-D gel electrophoresis ; Cells, Cultured ; Electrophoresis, Gel, Two-Dimensional - methods ; Fetal Blood - cytology ; Hematopoietic Stem Cells - metabolism ; Humans ; Mass spectrometry ; Molecular Weight ; Peptide Mapping - methods ; Proteome - analysis ; Proteome - chemistry ; Proteomics ; Stem cells</subject><ispartof>Biochemical and biophysical research communications, 2005-03, Vol.328 (4), p.968-972</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-5eadbcb2bfb56003a68d9fb63839c99d8c9374d96e655aec817cf8e6aac78e503</citedby><cites>FETCH-LOGICAL-c451t-5eadbcb2bfb56003a68d9fb63839c99d8c9374d96e655aec817cf8e6aac78e503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05000860$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15707972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zenzmaier, Christoph</creatorcontrib><creatorcontrib>Gesslbauer, Bernd</creatorcontrib><creatorcontrib>Grobuschek, Nina</creatorcontrib><creatorcontrib>Jandrositz, Anita</creatorcontrib><creatorcontrib>Preisegger, Karl-Heinz</creatorcontrib><creatorcontrib>Kungl, Andreas J.</creatorcontrib><title>Proteomic profiling of human stem cells derived from umbilical cord blood</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.</description><subject>2-D gel electrophoresis</subject><subject>Cells, Cultured</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Fetal Blood - cytology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Humans</subject><subject>Mass spectrometry</subject><subject>Molecular Weight</subject><subject>Peptide Mapping - methods</subject><subject>Proteome - analysis</subject><subject>Proteome - chemistry</subject><subject>Proteomics</subject><subject>Stem cells</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAURkVpaSaPP9BF0ao7u_falmxBNyE0DxhoFw1kJ_S4TjTYo1SyA_33tZmB7JLV3Zx7-DiMfUEoEVB-35XWJldWAKIELKFpPrANgoKiQmg-sg0AyKJS-HDCTnPeASA2Un1mJyhaaFVbbdjd7xQnimNw_DnFPgxh_8hjz5_m0ex5nmjkjoYhc08pvJDnfYojn0e7kM4M3MXkuR1i9OfsU2-GTBfHe8bur3_-ubottr9u7q4ut4VrBE6FIOOts5XtrZAAtZGdV72VdVcrp5TvnKrbxitJUghDrsPW9R1JY1zbkYD6jH07eJe9f2fKkx5DXjeaPcU5a9k2lRCdeBdEpWqBuBqrA-hSzDlRr59TGE36pxH0Wlrv9Fpar6U1oF5KL09fj_bZjuRfX45pF-DHAaAlxkugpLMLtHfkQyI3aR_DW_7_lmyPxw</recordid><startdate>20050325</startdate><enddate>20050325</enddate><creator>Zenzmaier, Christoph</creator><creator>Gesslbauer, Bernd</creator><creator>Grobuschek, Nina</creator><creator>Jandrositz, Anita</creator><creator>Preisegger, Karl-Heinz</creator><creator>Kungl, Andreas J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050325</creationdate><title>Proteomic profiling of human stem cells derived from umbilical cord blood</title><author>Zenzmaier, Christoph ; Gesslbauer, Bernd ; Grobuschek, Nina ; Jandrositz, Anita ; Preisegger, Karl-Heinz ; Kungl, Andreas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-5eadbcb2bfb56003a68d9fb63839c99d8c9374d96e655aec817cf8e6aac78e503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>2-D gel electrophoresis</topic><topic>Cells, Cultured</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Fetal Blood - cytology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Humans</topic><topic>Mass spectrometry</topic><topic>Molecular Weight</topic><topic>Peptide Mapping - methods</topic><topic>Proteome - analysis</topic><topic>Proteome - chemistry</topic><topic>Proteomics</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zenzmaier, Christoph</creatorcontrib><creatorcontrib>Gesslbauer, Bernd</creatorcontrib><creatorcontrib>Grobuschek, Nina</creatorcontrib><creatorcontrib>Jandrositz, Anita</creatorcontrib><creatorcontrib>Preisegger, Karl-Heinz</creatorcontrib><creatorcontrib>Kungl, Andreas J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zenzmaier, Christoph</au><au>Gesslbauer, Bernd</au><au>Grobuschek, Nina</au><au>Jandrositz, Anita</au><au>Preisegger, Karl-Heinz</au><au>Kungl, Andreas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic profiling of human stem cells derived from umbilical cord blood</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-03-25</date><risdate>2005</risdate><volume>328</volume><issue>4</issue><spage>968</spage><epage>972</epage><pages>968-972</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15707972</pmid><doi>10.1016/j.bbrc.2005.01.044</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2005-03, Vol.328 (4), p.968-972 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2-D gel electrophoresis Cells, Cultured Electrophoresis, Gel, Two-Dimensional - methods Fetal Blood - cytology Hematopoietic Stem Cells - metabolism Humans Mass spectrometry Molecular Weight Peptide Mapping - methods Proteome - analysis Proteome - chemistry Proteomics Stem cells |
| title | Proteomic profiling of human stem cells derived from umbilical cord blood |
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