Proteomic profiling of human stem cells derived from umbilical cord blood

CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or i...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-03, Vol.328 (4), p.968-972
Hauptverfasser:	Zenzmaier, Christoph, Gesslbauer, Bernd, Grobuschek, Nina, Jandrositz, Anita, Preisegger, Karl-Heinz, Kungl, Andreas J.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-D gel electrophoresis Cells, Cultured Electrophoresis, Gel, Two-Dimensional - methods Fetal Blood - cytology Hematopoietic Stem Cells - metabolism Humans Mass spectrometry Molecular Weight Peptide Mapping - methods Proteome - analysis Proteome - chemistry Proteomics Stem cells
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Zusammenfassung:	CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/j.bbrc.2005.01.044