A Field Validation of Plasma Metabolite Profiling to Assess Refueling Performance of Migratory Birds

Plasma metabolite profiling offers a potential means to assess stopover refueling performance of migratory birds from a single capture. However, this method has not previously been validated where site quality has been determined independently using analysis of capture data. We captured and blood sampled six passerine bird species refueling at known high‐quality (BASE) and low‐quality (TIP) sites at Long Point, Ontario, Canada. Plasma triglyceride, an indicator of fat deposition, was higher at the BASE in three early‐season species: the hermit thrush, the American robin, and the white‐throated sparrow. Plasma B‐OH‐butyrate, an indicator of fasting and lipid utilization, was lower at the BASE in the same three species. Plasma glycerol was lower at the BASE in American robins, and plasma phospholipid did not differ between sites. No metabolite suggested better conditions at the TIP in any species. Regression of size‐corrected mass on time of day also indicated better refueling performance at the BASE in some species, but metabolite profiling was generally more sensitive to site differences. The relationship between plasma glycerol and triglyceride was U‐shaped, indicating high glycerol production during both lipolysis (as was previously known) and rapid fat deposition. Our results confirm the validity of metabolite profiling to assess stopover habitat quality and individual performance in refueling migrants.