Regulation of embryo outgrowth by a morphogenic factor, epimorphin, in the mouse

Conceptus implantation to the uterine endometrium represents a complex series of events, including synchronized development of conceptus and uterus through up‐ and/or down‐regulation of numerous gene products. In a previous study using the DNA microarray technique, we had discovered evidence that increase in a transcript for mesenchymal morphogen, epimorphin, was noted as the conceptus attached to the matrix in vitro (Qin et al., 2003). In the present study, the expression and potential function of epimorphin in developing conceptuses was investigated through the use of reverse transcription‐polymerase chain reaction (RT‐PCR), whole mount in situ hybridization/immunohistochemistry, and in vitro blastocyst culture. RT‐PCR and in situ hybridization analysis revealed that epimorphin mRNA was expressed weakly in murine conceptuses during early developmental stages (1 cell to post‐adhesion blastocyst stages) and higher levels of epimorphin transcripts were observed in both inner cell mass (ICM) and trophectoderm of outgrowing blastocysts. Immunohistochemical analysis confirmed that epimorphin was localized in outgrowing trophoblast cells and ICM. Treating blastocysts in culture with a 115 kDa form of recombinant epimorphin promoted trophoblast outgrowth (P