Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis

Polymerase chain reaction (PCR) was used to detect the 47-kDa immunogen gene of Treponema pallidum in peripheral blood mononuclear cells (PBMCs), skin lesions, and serum, but less consistently in purified granulocytes or ejaculates of patients with manifest and latent syphilis. Therefore, skin lesio...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2005-02, Vol.51 (2), p.143-145
Hauptverfasser:	Kouznetsov, Alexander Vasilevich, Weisenseel, Peter, Trommler, Paul, Multhaup, Susanne, Prinz, Joerg Christoph
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Bacterial diseases of the genital system Bacteriology Base Sequence Biological and medical sciences Carrier Proteins - chemistry Carrier Proteins - isolation & purification DNA, Bacterial - analysis Female Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Lipoproteins - chemistry Lipoproteins - isolation & purification Male Medical sciences Microbiology Miscellaneous Molecular Sequence Data PBMCs Polymerase Chain Reaction Sensitivity and Specificity Skin - microbiology Syphilis Syphilis - diagnosis Syphilis, Cutaneous - diagnosis Syphilis, Latent - diagnosis Treponema pallidum Treponema pallidum - genetics Treponema pallidum - isolation & purification
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Zusammenfassung:	Polymerase chain reaction (PCR) was used to detect the 47-kDa immunogen gene of Treponema pallidum in peripheral blood mononuclear cells (PBMCs), skin lesions, and serum, but less consistently in purified granulocytes or ejaculates of patients with manifest and latent syphilis. Therefore, skin lesions and PBMCs may serve as the most reliable sources for a PCR-based diagnosis of syphilis.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2004.10.003