Rapid and Simple Quantification of Bacterial Cells by Using a Microfluidic Device

This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip...

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Veröffentlicht in:	Applied and Environmental Microbiology 2005-02, Vol.71 (2), p.1117-1121
Hauptverfasser:	Sakamoto, Chieko, Yamaguchi, Nobuyasu, Nasu, Masao
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Biotechnology Cell culture Colony Count, Microbial Culture Media Escherichia coli O157 - isolation & purification Flow Cytometry - instrumentation Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Methods Methods. Procedures. Technologies Microbiology Microfluidics - instrumentation Microscopy, Fluorescence Organic Chemicals Others Pseudomonas putida - isolation & purification Rivers - microbiology Staining and Labeling - methods Time Factors Various methods and equipments
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Beschreibung
Zusammenfassung:	This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.71.2.1117-1121.2005