Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds

A method of preparing titania‐coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus‐containing compounds, which have recently been attracting increasing attention in biochemical research. The titania‐coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho‐selectivity, which is a unique property of the titania surface. The material characteristics of titania‐coated monolithic silica were examined, and then resin‐clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania‐coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post‐genomic analyses.