The fabrication of protein chip based on surface plasmon resonance for detection of pathogens

Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of an...

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Veröffentlicht in:	Biosensors & bioelectronics 2005-03, Vol.20 (9), p.1847-1850
Hauptverfasser:	Oh, Byung-Keun, Lee, Woochang, Chun, Bum Suk, Bae, Young Min, Lee, Won Hong, Choi, Jeong-Woo
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - immunology Bacteria - isolation & purification Biocompatible Materials - chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Equipment Design Equipment Failure Analysis Escherichia coli Fundamental and applied biological sciences. Psychology Immunoassay - instrumentation Immunoassay - methods Legionella pneumophila Materials Testing Methods. Procedures. Technologies Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - immunology Pathogen Protein Array Analysis - instrumentation Protein Array Analysis - methods Protein chip Protein G Salmonella typhimurium Self-assembly technique Surface plasmon resonance Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods Various methods and equipments Yersinia enterocolitica
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Beschreibung
Zusammenfassung:	Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2004.05.010