Microsphere-Mediated Protein Delivery into Cells

Delivering the goods: By coupling proteins to varyingly sized polymeric microspheres, it is possible to deliver them to cells in an easy and effective way. For this study a fluorescent protein (EGFP) and a functional enzyme (β-galactosidase) were coupled to these particles. Evaluation of the cellula...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2009-06, Vol.10 (9), p.1453-1456
Hauptverfasser:	Sanchez-Martin, Rosario M, Alexander, Lois, Muzerelle, Mathilde, Cardenas-Maestre, Juan M, Tsakiridis, Anestis, Brickman, Joshua M, Bradley, Mark
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals beta-Galactosidase - administration & dosage beta-Galactosidase - chemistry beta-Galactosidase - metabolism carrier system Cell Line, Tumor fluorescent probes Fluorometry Green Fluorescent Proteins - administration & dosage Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism HeLa Cells Humans intracellular protein delivery Mice Microspheres nanoparticles Proteins - administration & dosage
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Beschreibung
Zusammenfassung:	Delivering the goods: By coupling proteins to varyingly sized polymeric microspheres, it is possible to deliver them to cells in an easy and effective way. For this study a fluorescent protein (EGFP) and a functional enzyme (β-galactosidase) were coupled to these particles. Evaluation of the cellular uptake after "beadfection" shows that the functionality and activity of these proteins were not adversely affected through coupling to the carrier system; this shows that their functional structure is retained.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200900136