Mechanistic and Structural Analysis of a Family 31 α-Glycosidase and Its Glycosyl-enzyme Intermediate

We have determined the first structure of a family 31 α-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-α-d-xylopyranosyl fluoride. Our 2.2-Å resolution structure shows an intimately associated hexamer w...

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