Mechanistic and Structural Analysis of a Family 31 α-Glycosidase and Its Glycosyl-enzyme Intermediate

We have determined the first structure of a family 31 α-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-α-d-xylopyranosyl fluoride. Our 2.2-Å resolution structure shows an intimately associated hexamer w...

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Veröffentlicht in:The Journal of biological chemistry 2005-01, Vol.280 (3), p.2105-2115
Hauptverfasser: Lovering, Andrew L., Lee, Seung Seo, Kim, Young-Wan, Withers, Stephen G., Strynadka, Natalie C.J.
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Sprache:eng
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Zusammenfassung:We have determined the first structure of a family 31 α-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-α-d-xylopyranosyl fluoride. Our 2.2-Å resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp416 to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian α-glucosidases lysosomal α-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M410468200