Direct Quantitation of Poly(ADP-Ribose) Polymerase (PARP) Activity as a Means to Distinguish Necrotic and Apoptotic Death in Cell and Tissue Samples

PARP‐angel of death. The PARP‐1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP‐1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, an...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2005-01, Vol.6 (1), p.53-55
Hauptverfasser:	Putt, Karson S, Beilman, Gregory J, Hergenrother, Paul J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Biological Assay - methods bioorganic chemistry enzymes NAD - analogs & derivatives NAD - analysis Necrosis PARP-1 Poly(ADP-ribose) Polymerases - analysis Poly(ADP-ribose) Polymerases - metabolism
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Beschreibung
Zusammenfassung:	PARP‐angel of death. The PARP‐1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP‐1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.
ISSN:	1439-4227 1439-7633 1439-4227
DOI:	10.1002/cbic.200400330