FT-Raman Spectroscopic Simultaneous Determination of Fructose and Glucose in Honey

A new method for mass percentage determination of fructose and glucose based on FT-Raman spectroscopy is evaluated with a standard HPLC-based method. FT-Raman spectra manipulation is done via the spectrometer software, and a PLS (partial least squares) method is developed with the TQ Analyst software (Ver 1. 1a). The simultaneous quantitative determination uses an input range from 1700 to 700 cm-1 without correction or baseline factors. The standards used in the PLS method are honey samples previously analyzed by HPLC to obtain their mass percentage concentrations in fructose and glucose. The returned results are statistically tested with those of the HPLC method. Both methods appear to score equally in terms of reproducibility. The honey content of the two sugars in total was found up to 40−74%. The honey samples content in fructose and glucose was determined by HPLC (24.1−42.9% and 16.2−33.1%, respectively) and FT-Raman (24.0−40.8% and 21.1−32.2%, respectively). Keywords: FT-Raman; honey; fructose; glucose; quantitative determination