Tissue microarray and immunohistochemistry as tools for evaluation of antibodies against Chlamydia-like bacteria

1 Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland 2 Center for Research on Intracellular Bacteria, Institute of Microbiology, University of Lausanne, Lausanne, Switzerland 3 Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland Correspondence Gilbert Greub gilbert.greub{at}chuv.ch Received January 8, 2009 Accepted March 6, 2009 Tissue microarray technology was used to establish immunohistochemistry protocols and to determine the specificity of new antisera against various Chlamydia -like bacteria for future use on formalin-fixed and paraffin-embedded tissues. The antisera exhibited strong reactivity against autologous antigen and closely related heterologous antigen, but no cross-reactivity with distantly related species.