Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid

1 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany 2 Berufsgenossenschaft Metall Nord Süd (BGM), Sachgebiet Biologische Noxen, D-55130 Mainz, Germany 3 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria Correspondence Peter Kämpfer peter.kaempfer{at}umwelt.uni-giessen.de A Gram-positive-staining, coccoid-shaped, oxidase-negative, non-spore-forming, non-motile bacterium, strain KSS-17Se T , was isolated from a metalworking fluid. On the basis of its major fatty acid (ai-C 15 : 0 ) and 16S rRNA gene sequence similarity, the strain grouped with Tessaracoccus bendigoensis and Tessaracoccus flavescens , sharing 95.3 and 97.4 % 16S rRNA gene sequence similarity with the respective type strains. Similarities with other established species of the genera Luteococcus , Propioniferax and Granulicoccus were lower than 95.5 %. The quinone system was characterized by the major menaquinone MK-9(H 4 ). In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and an unknown polar lipid were detected as major compounds. Additionally, three unknown glycolipids and minor amounts of phosphatidylethanolamine, two unknown aminolipids and two unknown polar lipids were detected. Phosphatidylinositol was present only in trace amounts. Predominant polyamines were spermine and spermidine. LL -Diaminopimelic acid was identified as the diagnostic diamino acid in the cell wall. The strain showed clear differences in phenotype (including chemotaxonomic features) from both Tessaracoccus species and members of the other above-mentioned genera. DNA–DNA hybridization between KSS-17Se T and T. bendigoensis Ben-106 T and T. flavescens SST-39 T yielded similarities of 15.1 and 21.0 %, respectively. It is evident that the organism represents a novel species, for which the name Tessaracoccus lubricantis sp. nov. is proposed. The type strain is KSS-17Se T (=DSM 19926 T =CCUG 55516 T ). Abbreviations: pNA, p -nitroanilide; pNP, p -nitrophenyl The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KSS-17Se T is FM178840 . The polar lipid profile of KSS-17Se T after two-dimensional TLC is available as supplementary material with the online version of this paper.