Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake

1 Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China 2 Graduate School of the Chinese Academy of Sciences, Beijing 100009, PR China 3 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China 4 Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China Correspondence Ren Lai rlai72{at}njau.edu.cn A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1 T , was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5–13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus , showing highest similarity to Bacillus macauensis JCM 13285 T (98.0 %). However, DNA–DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285 T (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C 15 : 0 (38.6 %) and anteiso-C 15 : 0 (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1 T (=KCTC 13181 T =CGMCC 1.6854 T ) should be classified as the type strain of a novel species of the genus Bacillus , for which the name Bacillus solisalsi sp. nov. is proposed. Abbreviations: AL, aminolipid; APL, aminophospholipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YC1 T is EU046268 . The TLC analysis of the polar lipid composition of the novel strain and B. macauensis and a transmission electron micrograph of cells of strain YC1 T are available as supplementary figures with the online version of this paper.