Rewritable Glycochips

We describe microarraying of carbohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization chemistries on plasmachemical deposited functional nanolayers. The commonly observed issue of nonspecific background binding of proteins is overcome by spotting carbohyd...

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Veröffentlicht in:	Journal of the American Chemical Society 2009-06, Vol.131 (22), p.7755-7761
Hauptverfasser:	Harris, L. G, Schofield, W. C. E, Doores, K. J, Davis, B. G, Badyal, J. P. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Aniline Compounds - chemistry Carbohydrates - chemistry Concanavalin A - chemistry Disulfides - chemistry Fluorescent Dyes - chemistry Glycols - chemistry Maltose - chemistry Microarray Analysis - methods Microscopy, Fluorescence Nanotechnology - methods Peanut Agglutinin - chemistry Protein Binding Spectroscopy, Fourier Transform Infrared Sulfhydryl Compounds - chemistry Sulfonic Acids - chemistry Vinyl Compounds - chemistry
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Beschreibung
Zusammenfassung:	We describe microarraying of carbohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization chemistries on plasmachemical deposited functional nanolayers. The commonly observed issue of nonspecific background binding of proteins is overcome by spotting carbohydrates through a protein-resistant overlayer yielding spatially localized interaction with a reactive functional underlayer.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja901294r