High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology

High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology

Thousands of kinase assays at one stroke are possible by using high‐content peptide microarrays. Such experiments deliver information regarding peptidic kinase substrates and accelerate the development of high‐throughput screening assays. Moreover, knowledge‐based selection of human sequences allows...

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Veröffentlicht in:Angewandte Chemie International Edition 2004-05, Vol.43 (20), p.2671-2674
Hauptverfasser: Schutkowski, Mike, Reimer, Ulf, Panse, Sören, Dong, Liying, Lizcano, Jose M., Alessi, Dario R., Schneider-Mergener, Jens
Format: Artikel
Sprache:eng
Schlagworte:
Automation
Binding Sites
Electrophoresis, Polyacrylamide Gel
enzymes
high-throughput screening
microarrays
peptides
Peptides - analysis
Peptides - metabolism
Phosphotransferases - analysis
Phosphotransferases - metabolism
Protein Array Analysis - methods
Substrate Specificity
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Beschreibung
Zusammenfassung:Thousands of kinase assays at one stroke are possible by using high‐content peptide microarrays. Such experiments deliver information regarding peptidic kinase substrates and accelerate the development of high‐throughput screening assays. Moreover, knowledge‐based selection of human sequences allows the reliable detection of downstream targets of protein kinases, as demonstrated for 3‐phosphoinositide‐dependent protein kinase.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200453900