Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes

[Display omitted] Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency ( k cat/ K m) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2005-01, Vol.15 (1), p.167-170
Hauptverfasser:	Kubo, Takanori, Takamori, Kengo, Kanno, Ken-ichi, Rumiana, Bakalova, Ohba, Hideki, Matsukisono, Miho, Akebiyama, Yasuo, Fujii, Masayuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences DNA, Catalytic - metabolism Gene Products, gag - genetics HIV-1 - genetics Hydrolysis Medical sciences Miscellaneous Pharmacology. Drug treatments RNA, Viral - chemistry RNA, Viral - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	[Display omitted] Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency ( k cat/ K m) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides responsible for nuclear cytoplasmic transport of proteins.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2004.10.009