In planta transformation of kenaf plants ( Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens

Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T 0 to T 1 plants, detection of the transgene in both T 0 and T 1 plants, and rescue of plasmids...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2004, Vol.98 (2), p.136-139
Hauptverfasser:	Kojima, Mineo, Shioiri, Hidenari, Nogawa, Masahiro, Nozue, Masayuki, Matsumoto, Daisuke, Wada, Asami, Saiki, Yumi, Kiguchi, Kenji
Format:	Artikel
Sprache:	eng
Schlagworte:	AGROBACTERIUM TUMEFACIENS Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Hibiscus cannabinus in planta transformation KENAF kenaf (Hibiscus cannabinus) nested PCR PCR rescue of T-DNA insertion locus TRANSGENIC PLANTS
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Zusammenfassung:	Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T 0 to T 1 plants, detection of the transgene in both T 0 and T 1 plants, and rescue of plasmids composed of T-DNA of the binary vector and flanking plant genomic DNA from T 1 plants.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/S1389-1723(04)70256-X