A Novel Carrier for Phanerochaete chrysosporium Immobilization

Phanerochaete chrysosporium BKMF-1767 cells were immobilized on different carriers. The optimum carrier according to adsorbed P. chrysosporium cells number (86.38%) was determined to be polystyrene foam, a novel carrier. The conditions for the immobilization of cells on polystyrene foam were optimiz...

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Veröffentlicht in:Artificial cells, blood substitutes, and immobilization biotechnology blood substitutes, and immobilization biotechnology, 2004, Vol.32 (4), p.563-574
Hauptverfasser: Ürek, Raziye Öztürk, Pazarlio lu, Nurdan Ka ikara
Format: Artikel
Sprache:eng
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Zusammenfassung:Phanerochaete chrysosporium BKMF-1767 cells were immobilized on different carriers. The optimum carrier according to adsorbed P. chrysosporium cells number (86.38%) was determined to be polystyrene foam, a novel carrier. The conditions for the immobilization of cells on polystyrene foam were optimized and determined as 50 rpm, 37°C, and 2 h. The results show that the adsorption of P. chrysosporium on polystyrene foam follows the Langmuir adsorption isotherm. High manganese peroxidase activity (421 U L) and dry mass (4.7 g L) were recovered from the batch mode polystyrene foam solid state fermentation system.
ISSN:1073-1199
1532-4184
DOI:10.1081/BIO-200039618