One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae
One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N....
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| Veröffentlicht in: | Journal of microbiological methods 2009-06, Vol.77 (3), p.323-325 |
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| container_title | Journal of microbiological methods |
| container_volume | 77 |
| creator | Lawung, Ratana Cherdtrakulkiat, Rungrot Charoenwatanachokchai, Angkana Nabu, Sunanta Suksaluk, Wanvisa Prachayasittikul, Virapong |
| description | One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty
Neisseria gonorrhoeae isolates, the penicillinase-producing
N. gonorrhoeae (PPNG), the high-level tetracycline-resistant
N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant
N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction. |
| doi_str_mv | 10.1016/j.mimet.2009.03.009 |
| format | Article |
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Neisseria gonorrhoeae isolates, the penicillinase-producing
N. gonorrhoeae (PPNG), the high-level tetracycline-resistant
N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant
N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2009.03.009</identifier><identifier>PMID: 19341768</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Drug Resistance, Bacterial ; Fundamental and applied biological sciences. Psychology ; Gonorrhea - microbiology ; Humans ; Microbiology ; Multiple antimicrobial resistance ; Multiplex PCR ; Neisseria gonorrhoeae ; Neisseria gonorrhoeae - drug effects ; Neisseria gonorrhoeae - genetics ; Neisseria gonorrhoeae - isolation & purification ; Neisseria gonorrhoeae - metabolism ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity</subject><ispartof>Journal of microbiological methods, 2009-06, Vol.77 (3), p.323-325</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-f9cfda3c390ce9109b68234f83156c53e0762be002dc0872b5b7005a2df3ce213</citedby><cites>FETCH-LOGICAL-c418t-f9cfda3c390ce9109b68234f83156c53e0762be002dc0872b5b7005a2df3ce213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2009.03.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21533941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19341768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawung, Ratana</creatorcontrib><creatorcontrib>Cherdtrakulkiat, Rungrot</creatorcontrib><creatorcontrib>Charoenwatanachokchai, Angkana</creatorcontrib><creatorcontrib>Nabu, Sunanta</creatorcontrib><creatorcontrib>Suksaluk, Wanvisa</creatorcontrib><creatorcontrib>Prachayasittikul, Virapong</creatorcontrib><title>One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty
Neisseria gonorrhoeae isolates, the penicillinase-producing
N. gonorrhoeae (PPNG), the high-level tetracycline-resistant
N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant
N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Drug Resistance, Bacterial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonorrhea - microbiology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Multiple antimicrobial resistance</subject><subject>Multiplex PCR</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - drug effects</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Neisseria gonorrhoeae - isolation & purification</subject><subject>Neisseria gonorrhoeae - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAUxC1ERZfCJ0BCvsAt4dlO4uTAAa34J1UtQnC2HOeZvlUSL7a3Et--XnYFNzjNwb958sww9kJALUB0b3b1QgvmWgIMNai6yCO2Eb2WVa_a4THbFEpXGoS8ZE9T2gGIVjX9E3YpBtUI3fUbZm9XrFLGPf-y_cp9iDzfIacJ10yenM0UVh48Xw5zpv2M3JaHhVwMI9mZR0yUsl1d8az8BikljGT5j7CGGO8CWnzGLrydEz4_6xX7_uH9t-2n6vr24-ftu-vKNaLPlR-cn6xyagCHg4Bh7HqpGt8r0XauVQi6kyMCyMlByTi2owZorZy8ciiFumKvT3f3Mfw8YMpmoeRwnu2K4ZBMp6VulZb_BSX03QAtFFCdwJI2pYje7CMtNv4yAsxxArMzvycwxwkMKFOkuF6ezx_GBae_nnPnBXh1Bmxydvax1EfpDyfLSGpojoHenjgsrd0TRpMcYal6oogumynQPz_yANzcphQ</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Lawung, Ratana</creator><creator>Cherdtrakulkiat, Rungrot</creator><creator>Charoenwatanachokchai, Angkana</creator><creator>Nabu, Sunanta</creator><creator>Suksaluk, Wanvisa</creator><creator>Prachayasittikul, Virapong</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae</title><author>Lawung, Ratana ; Cherdtrakulkiat, Rungrot ; Charoenwatanachokchai, Angkana ; Nabu, Sunanta ; Suksaluk, Wanvisa ; Prachayasittikul, Virapong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-f9cfda3c390ce9109b68234f83156c53e0762be002dc0872b5b7005a2df3ce213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Drug Resistance, Bacterial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonorrhea - microbiology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Multiple antimicrobial resistance</topic><topic>Multiplex PCR</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - drug effects</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Neisseria gonorrhoeae - isolation & purification</topic><topic>Neisseria gonorrhoeae - metabolism</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lawung, Ratana</creatorcontrib><creatorcontrib>Cherdtrakulkiat, Rungrot</creatorcontrib><creatorcontrib>Charoenwatanachokchai, Angkana</creatorcontrib><creatorcontrib>Nabu, Sunanta</creatorcontrib><creatorcontrib>Suksaluk, Wanvisa</creatorcontrib><creatorcontrib>Prachayasittikul, Virapong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawung, Ratana</au><au>Cherdtrakulkiat, Rungrot</au><au>Charoenwatanachokchai, Angkana</au><au>Nabu, Sunanta</au><au>Suksaluk, Wanvisa</au><au>Prachayasittikul, Virapong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>77</volume><issue>3</issue><spage>323</spage><epage>325</epage><pages>323-325</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty
Neisseria gonorrhoeae isolates, the penicillinase-producing
N. gonorrhoeae (PPNG), the high-level tetracycline-resistant
N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant
N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19341768</pmid><doi>10.1016/j.mimet.2009.03.009</doi><tpages>3</tpages></addata></record> |
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| ispartof | Journal of microbiological methods, 2009-06, Vol.77 (3), p.323-325 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Drug Resistance, Bacterial Fundamental and applied biological sciences. Psychology Gonorrhea - microbiology Humans Microbiology Multiple antimicrobial resistance Multiplex PCR Neisseria gonorrhoeae Neisseria gonorrhoeae - drug effects Neisseria gonorrhoeae - genetics Neisseria gonorrhoeae - isolation & purification Neisseria gonorrhoeae - metabolism Polymerase Chain Reaction - methods Sensitivity and Specificity |
| title | One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae |
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