One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N....

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Veröffentlicht in:	Journal of microbiological methods 2009-06, Vol.77 (3), p.323-325
Hauptverfasser:	Lawung, Ratana, Cherdtrakulkiat, Rungrot, Charoenwatanachokchai, Angkana, Nabu, Sunanta, Suksaluk, Wanvisa, Prachayasittikul, Virapong
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Drug Resistance, Bacterial Fundamental and applied biological sciences. Psychology Gonorrhea - microbiology Humans Microbiology Multiple antimicrobial resistance Multiplex PCR Neisseria gonorrhoeae Neisseria gonorrhoeae - drug effects Neisseria gonorrhoeae - genetics Neisseria gonorrhoeae - isolation & purification Neisseria gonorrhoeae - metabolism Polymerase Chain Reaction - methods Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2009.03.009