An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets
We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation...
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| Veröffentlicht in: | Lab on a chip 2009-01, Vol.9 (11), p.1576-1582 |
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| container_end_page | 1582 |
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| container_issue | 11 |
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| container_title | Lab on a chip |
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| creator | Hufnagel, Hansjörg Huebner, Ansgar Gülch, Carina Güse, Katharina Abell, Chris Hollfelder, Florian |
| description | We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates ( |
| doi_str_mv | 10.1039/b821695a |
| format | Article |
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The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates (<3%) and excellent growth properties in the device for up to 7 days for CHO-K1 cells. To demonstrate that key procedures from the repertoire of cell biology are possible in this format, transfection of a reporter gene (encoding green fluorescent protein) was carried out. The modular design enables efficient detachment and recollection of cells and allows assessment of the success of transfection achieved on-chip. The transfection levels (20%) are comparable to standard large scale procedures and more than 500 cells could be transfected. Finally, cells are transferred into microfluidic microdoplets, where in principle a wide range of subsequent assays can be carried out at the single cell level in droplet compartments. 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The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates (<3%) and excellent growth properties in the device for up to 7 days for CHO-K1 cells. To demonstrate that key procedures from the repertoire of cell biology are possible in this format, transfection of a reporter gene (encoding green fluorescent protein) was carried out. The modular design enables efficient detachment and recollection of cells and allows assessment of the success of transfection achieved on-chip. The transfection levels (20%) are comparable to standard large scale procedures and more than 500 cells could be transfected. Finally, cells are transferred into microfluidic microdoplets, where in principle a wide range of subsequent assays can be carried out at the single cell level in droplet compartments. The procedures developed for this modular device layout further demonstrate that commonly used methods in cell biology involving mammalian cells can be reliably scaled down to allow single cell investigations in picolitre volumes.</description><subject>Animals</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Cells, Immobilized - physiology</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Equipment Design</subject><subject>Genes, Reporter</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microtechnology - instrumentation</subject><subject>Transfection</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0URB4g5Qsir6JsBvx-sIuiQJAisYF1q9pdBkfu9mB3j5SfyDczk5nAMquqxdG5VbqEnHP2kTPpP_VOcOM1vCEnXFm5Ytz5o3-7t8fktLUHxrhWxr0jx9wr7ZzRJ-TpeqJpmvFXhRkHGjBnGpY8LxVphp6WiQINv9P6Mx3LsGSodEyhlgE3KWCjsdRnPm1gTlu4RDrCOEJOMD3bGoVpoAPmtMH6uMsqe0PMSxpSOOhqWWec23vyNkJu-OEwz8jPL7c_bu5W99-_fru5vl8FqeS8Er0IwJ0WTIIDxY3A2EfdA7PcG-fBe22NFFEgeGNDb1AH5ng_KGNjEPKMXO6961r-LNjmbkxtdy5MWJbWGSusFOZ1UGomlLL6VVBwbq3iO-PVHty-3VrF2K1rGqE-dpx1uza7lza36MXBufQjDv_BQ33yLxyenEQ</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Hufnagel, Hansjörg</creator><creator>Huebner, Ansgar</creator><creator>Gülch, Carina</creator><creator>Güse, Katharina</creator><creator>Abell, Chris</creator><creator>Hollfelder, Florian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets</title><author>Hufnagel, Hansjörg ; Huebner, Ansgar ; Gülch, Carina ; Güse, Katharina ; Abell, Chris ; Hollfelder, Florian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-2b2ca185203a8a4162efbf5ba0719689a9957632f2ea967cb6e5c081bd467fc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Cells, Immobilized - physiology</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Equipment Design</topic><topic>Genes, Reporter</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microtechnology - instrumentation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hufnagel, Hansjörg</creatorcontrib><creatorcontrib>Huebner, Ansgar</creatorcontrib><creatorcontrib>Gülch, Carina</creatorcontrib><creatorcontrib>Güse, Katharina</creatorcontrib><creatorcontrib>Abell, Chris</creatorcontrib><creatorcontrib>Hollfelder, Florian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hufnagel, Hansjörg</au><au>Huebner, Ansgar</au><au>Gülch, Carina</au><au>Güse, Katharina</au><au>Abell, Chris</au><au>Hollfelder, Florian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>9</volume><issue>11</issue><spage>1576</spage><epage>1582</epage><pages>1576-1582</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates (<3%) and excellent growth properties in the device for up to 7 days for CHO-K1 cells. To demonstrate that key procedures from the repertoire of cell biology are possible in this format, transfection of a reporter gene (encoding green fluorescent protein) was carried out. The modular design enables efficient detachment and recollection of cells and allows assessment of the success of transfection achieved on-chip. The transfection levels (20%) are comparable to standard large scale procedures and more than 500 cells could be transfected. Finally, cells are transferred into microfluidic microdoplets, where in principle a wide range of subsequent assays can be carried out at the single cell level in droplet compartments. 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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2009-01, Vol.9 (11), p.1576-1582 |
| issn | 1473-0197 1473-0189 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Cell Culture Techniques - instrumentation Cell Proliferation Cell Survival Cells, Immobilized - physiology CHO Cells Cricetinae Cricetulus Equipment Design Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Microfluidic Analytical Techniques - instrumentation Microtechnology - instrumentation Transfection |
| title | An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets |
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