An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets

We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation...

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Veröffentlicht in:	Lab on a chip 2009-01, Vol.9 (11), p.1576-1582
Hauptverfasser:	Hufnagel, Hansjörg, Huebner, Ansgar, Gülch, Carina, Güse, Katharina, Abell, Chris, Hollfelder, Florian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques - instrumentation Cell Proliferation Cell Survival Cells, Immobilized - physiology CHO Cells Cricetinae Cricetulus Equipment Design Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Microfluidic Analytical Techniques - instrumentation Microtechnology - instrumentation Transfection
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Beschreibung
Zusammenfassung:	We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates (
ISSN:	1473-0197 1473-0189
DOI:	10.1039/b821695a