Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry
Enzyme‐filled stable polymeric capsules (polymersomes) that are permeable to low‐molecular‐weight (pro‐fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build‐up of fluorescent product that is p...
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| Veröffentlicht in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2009-05, Vol.5 (10), p.1138-1143 |
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| creator | Nallani, Madhavan Woestenenk, Rob de Hoog, Hans‐Peter M. van Dongen, Stijn F. M. Boezeman, Jan Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. van Hest, Jan C. M. |
| description | Enzyme‐filled stable polymeric capsules (polymersomes) that are permeable to low‐molecular‐weight (pro‐fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build‐up of fluorescent product that is prevented from leaking out by co‐encapsulation of a trapping agent. |
| doi_str_mv | 10.1002/smll.200801204 |
| format | Article |
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Enzymatic activity is screened by the build‐up of fluorescent product that is prevented from leaking out by co‐encapsulation of a trapping agent.</description><identifier>ISSN: 1613-6810</identifier><identifier>EISSN: 1613-6829</identifier><identifier>DOI: 10.1002/smll.200801204</identifier><identifier>PMID: 19235803</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag</publisher><subject>Catalysis ; enzymes ; Flow Cytometry ; Fluoresceins - chemistry ; Light ; nanoreactors ; Nanostructures - chemistry ; Polymers - chemistry ; polymersomes ; Scattering, Radiation</subject><ispartof>Small (Weinheim an der Bergstrasse, Germany), 2009-05, Vol.5 (10), p.1138-1143</ispartof><rights>Copyright © 2009 Wiley‐VCH Verlag GmbH & Co. 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Enzymatic activity is screened by the build‐up of fluorescent product that is prevented from leaking out by co‐encapsulation of a trapping agent.</description><subject>Catalysis</subject><subject>enzymes</subject><subject>Flow Cytometry</subject><subject>Fluoresceins - chemistry</subject><subject>Light</subject><subject>nanoreactors</subject><subject>Nanostructures - chemistry</subject><subject>Polymers - chemistry</subject><subject>polymersomes</subject><subject>Scattering, Radiation</subject><issn>1613-6810</issn><issn>1613-6829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4MoTqdXj9KTt878bNPjKG4K9QdMzyFNU6mky0wyR_57Oza24w6P9-B93hfeB4A7BCcIQvzoe2MmGEIOEYb0DFyhDJE047g4P8wIjsC19z8QEoRpfglGqMCEcUiuwHxhXeiW30kpgzQxdEoaE5OpCt2fTj6sib123vY6eZNL67RUwTqf1DGZGbtJyhiGXXDxBly00nh9u-9j8DV7-iyf0-p9_lJOq1RRRGmKswxikumsrhFUijU0Jy3DtcZFXmDEeAM1bTjhnJG25RyrVrds-LRWUmPWkDF42OWunP1dax9E33mljZFLbddeZDlmGFFyEiR08DLUAE52oHLWe6dbsXJdL10UCIqtY7F1LA6Oh4P7ffK67nVzxPdSB6DYAZvO6HgiTixeq-oY_g-HPoiN</recordid><startdate>20090518</startdate><enddate>20090518</enddate><creator>Nallani, Madhavan</creator><creator>Woestenenk, Rob</creator><creator>de Hoog, Hans‐Peter M.</creator><creator>van Dongen, Stijn F. 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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Catalysis enzymes Flow Cytometry Fluoresceins - chemistry Light nanoreactors Nanostructures - chemistry Polymers - chemistry polymersomes Scattering, Radiation |
| title | Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry |
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