Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry

Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry

Enzyme‐filled stable polymeric capsules (polymersomes) that are permeable to low‐molecular‐weight (pro‐fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build‐up of fluorescent product that is p...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2009-05, Vol.5 (10), p.1138-1143
Hauptverfasser: Nallani, Madhavan, Woestenenk, Rob, de Hoog, Hans‐Peter M., van Dongen, Stijn F. M., Boezeman, Jan, Cornelissen, Jeroen J. L. M., Nolte, Roeland J. M., van Hest, Jan C. M.
Format: Artikel
Sprache:eng
Schlagworte:
Catalysis
enzymes
Flow Cytometry
Fluoresceins - chemistry
Light
nanoreactors
Nanostructures - chemistry
Polymers - chemistry
polymersomes
Scattering, Radiation
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Zusammenfassung:Enzyme‐filled stable polymeric capsules (polymersomes) that are permeable to low‐molecular‐weight (pro‐fluorescent) substrates can be sorted based on the activity of encapsulated enzymes using flow cytometry (see image). Enzymatic activity is screened by the build‐up of fluorescent product that is prevented from leaking out by co‐encapsulation of a trapping agent.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200801204