Metal-polymer nanocomposites for integrated microfluidic separations and surface enhanced Raman spectroscopic detection

The widespread development of microfluidics (μfluidics) has allowed the extension of efficient separations, fluid handling, and hyphenation with many detection modes to a small, portable, highly controllable physico‐chemical platform. Surface enhanced Raman spectroscopy (SERS) offers the powerful advantage of obtaining vibrational spectroscopic information about analytes in an aqueous matrix with negligible background. The mating of electrophoretic separations with vibrational spectroscopy on a μfluidic device will allow the chromatographic efficiency of capillary electrophoresis (CE) with the unequivocal analyte “fingerprinting” capability of detailed structural information. By utilizing SERS as a means of detection, this work promises to yield redress for the hindrances of electrophoretic separations, including uncertainty in analyte band identification due to changing migration times as well as compromised detection sensitivity for non‐fluorescent analytes. Our work represents the first steps toward developing CE‐SERS on a μfluidic platform with a region of novel metal‐pliable polymer nanocomposite SERS substrate fabricated directly into the device. The device fabrication material has been extensively employed by the μfluidics community for over five years. SERS detection can be achieved in real time or after the separations, with on‐column laser‐induced fluorescence employed as a secondary detection mode used for confirmation of efficiencies and band locations.