Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate
Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, α-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces.1,2 We intercal...
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| Veröffentlicht in: | Analytical Sciences 2004, Vol.20(12), pp.1635-1638 |
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| creator | PARK, Sejin CHUNG, Taek Dong KANG, Sun Kil JEONG, Ran-A BOO, Hankil KIM, Hee Chan |
| description | Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, α-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces.1,2 We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0 - 25 mM range and the sensitivity of 0.14 nA mM-1 at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration. |
| doi_str_mv | 10.2116/analsci.20.1635 |
| format | Article |
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Reportedly, α-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces.1,2 We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0 - 25 mM range and the sensitivity of 0.14 nA mM-1 at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.20.1635</identifier><identifier>PMID: 15636507</identifier><language>eng</language><publisher>Japan: The Japan Society for Analytical Chemistry</publisher><subject>Biosensing Techniques ; Electrochemistry ; Electrodes ; Enzymes, Immobilized - chemistry ; Glucose - analysis ; Glucose Oxidase - chemistry ; Kinetics ; Microscopy, Electron, Scanning ; Platinum - chemistry ; Polyvinyl Alcohol - chemistry ; Sensitivity and Specificity ; Zirconium - chemistry</subject><ispartof>Analytical Sciences, 2004, Vol.20(12), pp.1635-1638</ispartof><rights>2004 by The Japan Society for Analytical Chemistry</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-555a6072c299f68a58624e17acbe32459d26c3c23dfc16b7ea477e4e4e1366253</citedby><cites>FETCH-LOGICAL-c550t-555a6072c299f68a58624e17acbe32459d26c3c23dfc16b7ea477e4e4e1366253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15636507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PARK, Sejin</creatorcontrib><creatorcontrib>CHUNG, Taek Dong</creatorcontrib><creatorcontrib>KANG, Sun Kil</creatorcontrib><creatorcontrib>JEONG, Ran-A</creatorcontrib><creatorcontrib>BOO, Hankil</creatorcontrib><creatorcontrib>KIM, Hee Chan</creatorcontrib><title>Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate</title><title>Analytical Sciences</title><addtitle>Anal Sci</addtitle><description>Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. 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The sensors, as made, were stable for more than 3 days within a limited deterioration.</description><subject>Biosensing Techniques</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Glucose - analysis</subject><subject>Glucose Oxidase - chemistry</subject><subject>Kinetics</subject><subject>Microscopy, Electron, Scanning</subject><subject>Platinum - chemistry</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Sensitivity and Specificity</subject><subject>Zirconium - chemistry</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtL9DAUQIMoOj7W7qQguOuY3DzaWap8vhAU1I2bkKZ3nAxtMyYtfPrrbZmqKIEEbk4O4RByyOgUGFOnpjFVtG4KdMoUlxtkwrjIUwChNsmEzhhNFRd0h-zGuKSUQQ6wTXaYVFxJmk3I7VXVWR8xecQm-pCcm4hl4pvka37_35X9LLmpa1-4yn3018V78uKC9Y3r6uRh4eNqYVrcJ1vz_jd4MJ575Pny39PFdXp3f3VzcXaXWilpm0opjaIZWJjN5io3MlcgkGXGFshByFkJynILvJxbpooMjcgyFP1iXCmQfI-crL2r4N86jK2uXbRYVaZB30WtMgCa87wHj_-AS9-FoZhmQuRSZFQOutM1ZYOPMeBcr4KrTXjXjOohsh4ja6B6iNy_OBq9XVFj-cOPVXvgcg0sY2te8RswoXW2wl9CGPfB_A3YhQkaG_4J2UaQ9w</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>PARK, Sejin</creator><creator>CHUNG, Taek Dong</creator><creator>KANG, Sun Kil</creator><creator>JEONG, Ran-A</creator><creator>BOO, Hankil</creator><creator>KIM, Hee Chan</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate</title><author>PARK, Sejin ; CHUNG, Taek Dong ; KANG, Sun Kil ; JEONG, Ran-A ; BOO, Hankil ; KIM, Hee Chan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-555a6072c299f68a58624e17acbe32459d26c3c23dfc16b7ea477e4e4e1366253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biosensing Techniques</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Glucose - analysis</topic><topic>Glucose Oxidase - chemistry</topic><topic>Kinetics</topic><topic>Microscopy, Electron, Scanning</topic><topic>Platinum - chemistry</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Zirconium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PARK, Sejin</creatorcontrib><creatorcontrib>CHUNG, Taek Dong</creatorcontrib><creatorcontrib>KANG, Sun Kil</creatorcontrib><creatorcontrib>JEONG, Ran-A</creatorcontrib><creatorcontrib>BOO, Hankil</creatorcontrib><creatorcontrib>KIM, Hee Chan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PARK, Sejin</au><au>CHUNG, Taek Dong</au><au>KANG, Sun Kil</au><au>JEONG, Ran-A</au><au>BOO, Hankil</au><au>KIM, Hee Chan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate</atitle><jtitle>Analytical Sciences</jtitle><addtitle>Anal Sci</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>20</volume><issue>12</issue><spage>1635</spage><epage>1638</epage><pages>1635-1638</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. 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| subjects | Biosensing Techniques Electrochemistry Electrodes Enzymes, Immobilized - chemistry Glucose - analysis Glucose Oxidase - chemistry Kinetics Microscopy, Electron, Scanning Platinum - chemistry Polyvinyl Alcohol - chemistry Sensitivity and Specificity Zirconium - chemistry |
| title | Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate |
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