Adult Rat Odontoblasts Lack Noxious Thermal Sensitivity

Dentin hypersensitivity is a common symptom treated in the dental clinic, yet the underlying cellular and molecular mechanisms are not clear. We hypothesized that odontoblasts detect noxious thermal stimuli by expressing temperature-sensing molecules, and investigated whether temperature-activated T...

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Veröffentlicht in:Journal of dental research 2009-04, Vol.88 (4), p.328-332
Hauptverfasser: Yeon, K.Y., Chung, G., Shin, M.S., Jung, S.J., Kim, J.S., Oh, S.B.
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Sprache:eng
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Zusammenfassung:Dentin hypersensitivity is a common symptom treated in the dental clinic, yet the underlying cellular and molecular mechanisms are not clear. We hypothesized that odontoblasts detect noxious thermal stimuli by expressing temperature-sensing molecules, and investigated whether temperature-activated TRP channels (thermo-TRP channels), which are known to initiate temperature signaling, mediate temperature sensing in odontoblasts. mRNA expression of dentin sialophosphoprotein and collagenase type 1, odontoblast-specific proteins, was shown in acutely isolated adult rat odontoblasts by single-cell RT-PCR, while TRPV1, TRPV2, TRPM8, and TRPA1 were not detected. Application of noxious temperatures of 42°C and 12°C, as well as capsaicin, menthol, and icilin, agonists of thermo-TRP channels, failed to increase intracellular calcium concentration. Immunohistochemical study also revealed no expression of TRPV1. Thus, it is unlikely that odontoblasts serve as thermal sensors in teeth via thermo-TRP channels.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034509334100