Analysis of the Poplar Phloem Proteome and Its Response to Leaf Wounding

Phloem exudate collected from hybrid poplar (Populus trichocarpa × Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P < 0.0...

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Veröffentlicht in:	Journal of proteome research 2009-05, Vol.8 (5), p.2341-2350
Hauptverfasser:	Dafoe, Nicole J, Zamani, Arezoo, Ekramoddoullah, Abul K. M, Lippert, Dustin, Bohlmann, Jörg, Constabel, C. Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Gene Library Immunoblotting Microscopy, Fluorescence Phloem - genetics Phloem - metabolism Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - classification Plant Proteins - genetics Plant Proteins - metabolism Populus - genetics Populus - metabolism Proteome - classification Proteome - genetics Proteome - metabolism Proteomics - methods Stress, Mechanical Tandem Mass Spectrometry
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Zusammenfassung:	Phloem exudate collected from hybrid poplar (Populus trichocarpa × Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P < 0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protein. This is the first characterization of a phloem proteome from a tree species.
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr800968r