Evaluation of nano-liquid chromatography-tandem mass spectrometry in a column switching setup for the absolute quantification of peptides in the picomolar range

A standard nanospray-liquid chromatography-tandem mass spectrometry system in a column switching setup for the absolute quantification of leucine-enkephalin was evaluated. Analytes were loaded on a C18 trapping column and back-flushed in the 75 microm analytical column. Quantification was performed...

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Veröffentlicht in:	Journal of Chromatography A 2004-11, Vol.1058 (1-2), p.113-119
Hauptverfasser:	Sinnaeve, Bart A, Van Bocxlaer, Jan F
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Liquid - methods Mass Spectrometry - methods Microscopy, Electron, Scanning Nanotechnology Peptides - analysis
Online-Zugang:	Volltext
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